Presence of thyroxine in eggs and changes in its content during early development of chum salmon, Oncorhynchus keta.

@article{Tagawa1987PresenceOT,
  title={Presence of thyroxine in eggs and changes in its content during early development of chum salmon, Oncorhynchus keta.},
  author={Masatomo Tagawa and Tetsuya Hirano},
  journal={General and comparative endocrinology},
  year={1987},
  volume={68 1},
  pages={129-35}
}
In order to examine the role of thyroid hormones during salmonid development, techniques were developed for quantitative extraction of thyroxine from eggs, whole embryos, and alevins of chum salmon (Oncorhynchus keta) at various stages of development. Frozen eggs, embryos, alevins, or fry were homogenized in ice-cold methanol. The homogenate was centrifuged, and the supernatant was washed with a mixture of chloroform and 0.05% CaCl2. The aqueous layer was lyophilized, and the residue was… CONTINUE READING