Presence of the molybdenum-cofactor in nitrate reductase-deficient mutant cell lines of Nicotiana tabacum

  title={Presence of the molybdenum-cofactor in nitrate reductase-deficient mutant cell lines of Nicotiana tabacum},
  author={Ralf R. Mendel and Zerekbai A. Alikulov and Nikolai P. Lvov and Andreas M{\"u}ller},
  journal={Molecular and General Genetics MGG},
Nicotiana tabacum mutant cell cultures lacking nitrate reductase activity were assayed for the presence of the molybdenum-cofactor using its ability to restore NADPH-nitrate reductase activity in extracts of Neurospora crassa nit-1 mycelia. The molybdenum-cofactor of the tobacco wild-type line was shown to complement efficiently the N. crassa nit-1 mutant in vitro. The molybdenum-cofactor seems to exist in a bound form, as acid-treatment was required for release of cofactor activity. Molybdate… CONTINUE READING
13 Citations
25 References
Similar Papers


Publications citing this paper.
Showing 1-10 of 13 extracted citations


Publications referenced by this paper.
Showing 1-10 of 25 references

Comparative characterization of nitrate reductase from wild-type and molybdenum cofactor-defective cell cultures of Nicotiana tabacurn

  • RR Mendel, AJ Mfiller
  • Plant Sci Lett 18:277-288
  • 1980

Isolation of cofactor from molybdo-enzymes: nitrate reductase of lupin bacteroids and xanthinoxidase of milk

  • ZA Alikulov, NP Lvov, SH SBurikhanov, VL Kretovich
  • Izvest Acad Sci USSR, Ser Biol
  • 1980
1 Excerpt

Genetic and biochemical analysis of mutants affected in nitrate reduction in Rhizobium meliloti

  • Gy Kiss, E Vincze, Zs Kalman, T Forrai, A Kondorosi
  • J Gen Microbiol
  • 1979
1 Excerpt

Isolation and characterization of cell lines of Nicotiana tabacum lacking nitrate reductase

  • AJ Mtiller, R Grafe
  • Mol Gen Genet
  • 1978
1 Excerpt

Similar Papers

Loading similar papers…