Presence of heparan sulfate in the glomerular basement membrane.

  title={Presence of heparan sulfate in the glomerular basement membrane.},
  author={Yashpal S. Kanwar and Marilyn Gist Farquhar},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={76 3},
The glomerular basement membrane was subjected to digestion with specific enzymes to determine the chemical nature (sialoglycoproteins, collagenous peptides, or glycosaminoglycans) of the anionic sites previously demonstrated in the laminae rarae. Enzyme digestion was carried out both in situ and in vitro. Kidneys were perfused in situ with enzyme solutions followed by perfusion with fixative containing the cationic dye, ruthenium red, to detect the anionic sites. Glomerular basement membranes… CONTINUE READING
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