Presence in the 'silent' terminus region of the Escherichia coli K12 chromosome of cryptic gene(s) encoding a new nitrate reductase.

@article{Bonnefoy1987PresenceIT,
  title={Presence in the 'silent' terminus region of the Escherichia coli K12 chromosome of cryptic gene(s) encoding a new nitrate reductase.},
  author={Violaine Bonnefoy and Jean François Burini and G{\'e}rard Giordano and M. C. Pascal and Marc Chippaux},
  journal={Molecular microbiology},
  year={1987},
  volume={1 2},
  pages={143-50}
}
A cosmid complementing narG mutants defective in nitrate reductase activity was isolated from a genomic library of Escherichia coli. The restriction map of the insert differed from that of the narGHI operon. The new enzyme, termed NarZ, required molybdenum for activity. The expression of narZ was not affected by the factors controlling narGHI. Insertion mutations indicated that the narZ locus covered about 8 kb of DNA; narZ is located at 32.5 U on the chromosome, in the cotransduction gap near… CONTINUE READING

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