Preparing well-oriented sols of straight bacterial flagellar filaments for X-ray fiber diffraction.

Abstract

Well-oriented sols of straight bacterial flagellar filaments have been obtained by preparing reconstituted flagellar filaments with an appropriate length distribution and choosing appropriate solvent conditions. An average filament length of 300 to 500 nm and the use of solvents with very low concentrations of salt has allowed us to prepare highly fluid… (More)

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