Preparative steps necessary for the accurate measurement of malondialdehyde by high-performance liquid chromatography.

@article{Lepage1991PreparativeSN,
  title={Preparative steps necessary for the accurate measurement of malondialdehyde by high-performance liquid chromatography.},
  author={Guy Lepage and Gabriela Mu{\~n}oz and Jos{\'e}e Champagne and Claude Roy},
  journal={Analytical biochemistry},
  year={1991},
  volume={197 2},
  pages={277-83}
}
The need for a more specific, reliable, and reproducible technique for the measurement of malondialdehyde (MDA) has prompted modifications of currently available methods based on the formation and recovery of the complex between MDA and thiobarbituric acid (TBA). To 500 microliters of plasma or to 300 mg of liver homogenate, 2 ml of H2O and 500 microliters of 0.5% butylated hydroxytoluene in methanol were added to prevent further formation of MDA. Precipitation of proteins carried out with 200… CONTINUE READING
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