Preparative isolation of middle molecular weight fractions from the hemofiltrate of patients with chronic uremia.

@article{Brunner1978PreparativeIO,
  title={Preparative isolation of middle molecular weight fractions from the hemofiltrate of patients with chronic uremia.},
  author={Herwig Brunner and H. B. Mann and Ursula Essers and R Schultheis and Thomas Byrne and Robert Heintz},
  journal={Artificial organs},
  year={1978},
  volume={2 4},
  pages={375-7}
}
Fractions in the molecular weight range of the so-called middle molecules (500--3000 daltons) were isolated on a preparative scale from hemofiltrate of patients. Hemofiltration was performed with an RP-6 dialyzer using a predilution technique. The hemofiltrate was concentrated and desalinated by reverse osmosis with membranes with a normal cut-off of 500 daltons. The retentate was freeze-dried, redissolved in volatile buffer and fractionated on Sephadex G-15 macrocolumns. The middle molecule… CONTINUE READING