Preparative isolation of adult human liver metallothionein isoforms.

  title={Preparative isolation of adult human liver metallothionein isoforms.},
  author={Juan Hidalgo and A D Dingman and Justine S. Garvey},
  journal={Revista espanola de fisiologia},
  volume={45 3},
Preparative human liver metallothionein (MT) isolation is described. MT was saturated with cadmium to follow MT purification spectrophotometrically instead of by metal content and to increase the stability of the protein. A concentrated, MT-rich fraction of the liver cytosol was prepared by selective organic solvent (acetone or acetone/methanol) fractionation. Conventional gel filtration and ion-exchange chromatographies resolved two MT isoforms, MT-I and MT-II. When needed, purification of MT… CONTINUE READING