Preparative acrylamide electrophoresis: a single gel system.

@article{Gordon1967PreparativeAE,
  title={Preparative acrylamide electrophoresis: a single gel system.},
  author={A. H. Gordon and L. Louis},
  journal={Analytical biochemistry},
  year={1967},
  volume={21 2},
  pages={
          190-200
        }
}
1. 1. An apparatus is described for continuous elution of substances as they are separated by electrophoresis in acrylamide gel. The elution buffer used for this purpose is pumped inward across the base of a cylinder of gel and then passes upward and out via a central capillary. 2. 2. Proteins and other large molecules are prevented from passing into the electrode buffer by means of a cellophane membrane. The membrane is rigidly mounted on a Perspex grid which forms part of an elution cell… Expand
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