Preparative Scale Purification of Recombinant Proteins to Clinical Grade by Isotachophoresis

@article{Stowers1995PreparativeSP,
  title={Preparative Scale Purification of Recombinant Proteins to Clinical Grade by Isotachophoresis},
  author={Anthony W. Stowers and Kevin J. Spring and A Tova Saul},
  journal={Bio/Technology},
  year={1995},
  volume={13},
  pages={1498-1503}
}
An electrophoretic procedure based on isotachophoresis has been developed for protein purification on a preparative scale in the 10 to 500 mg range. The system is simple, uses well understood physical properties, does not need ampholyte spacers and is able to produce sterile products of clinical grade. We demonstrate the applicability of this apparatus for the purification of denatured recombinant proteins and complex mixtures of proteins. The system may also be used for both cationic and… CONTINUE READING