Preparative Purification of Delphinidin 3-0-sambubioside from Roselle (Hibiscus sabdariffa L.) Petals by fast Centrifugation Partition Chromatography

@article{Kouakou2010PreparativePO,
  title={Preparative Purification of Delphinidin 3-0-sambubioside from Roselle (Hibiscus sabdariffa L.) Petals by fast Centrifugation Partition Chromatography},
  author={H. Kouakou and L. K. Kouakou and A. D{\'e}cendit and A. Badoc and Gregory Da-Costa and J. M{\'e}rillon and P. W. Teguo},
  journal={Journal of Advances in Chemistry},
  year={2010},
  volume={6},
  pages={999-1004}
}
Delphinidin 3- 0 -sambubioside, a Hibiscus anthocyanin, was isolated from MeOH/TFA dried flower of H. sabdariffa . Its purification on preparative scale was obtained by centrifugal partition chromatography (CPC) using the ternary biphasic solvent systems composed of ethyl acetate/1-butanol/water, acidified by 0.1% of TFA. Stationary phase was ethyl acetate/1-butanol/water (5:5:90; v/v). We tested 4 mobile phases and found that the system acetate/1-butanol/water (40:46:14; v/v) was the best to… Expand

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References

SHOWING 1-10 OF 29 REFERENCES
Preparative separation of anthocyanins by gradient elution centrifugal partition chromatography
Abstract Centrifugal partition chromatography (CPC) allowed purification of anthocyaanins from Champagne vintage by-products (Vitis vinifera) and from blackcurrant (Ribes ni9grum L.). In the firstExpand
Study on furundu, a traditional Sudanese fermented roselle (Hibiscus sabdariffa L.) seed: effect on in vitro protein digestibility, chemical composition, and functional properties of the total proteins.
TLDR
The results show that cooking followed by fermentation affects proteins solubility in water and 1 M NaCl, and the foaming capacity from the flour of raw seed decreased as a result of cooking. Expand
Cyanidin and Malvidin from Oryza sativa cv. Heugjinjubyeo mediate cytotoxicity against human monocytic leukemia cells by arrest of G(2)/M phase and induction of apoptosis.
  • J. Hyun, H. Chung
  • Chemistry, Medicine
  • Journal of agricultural and food chemistry
  • 2004
Oryza sativa cv. Heugjinjubyeo (Gramineae), anthocyanin-pigmented rice, having dark purple grains, is known broadly as enriched rice with an improved taste. Two bioactive compounds were isolated fromExpand
Thermal kinetic degradation of anthocyanins in a roselle (Hibiscus sabdariffa L. cv. ‘Criollo’) infusion
Summary The degradation kinetics of anthocyanins in a Roselle infusion (Hibiscus sabdariffa L.), as assessed by visible absorption spectroscopy (absorbance at 520 nm), was verified (60–100 °C).Expand
Protective effect of Hibiscus anthocyanins against tert-butyl hydroperoxide-induced hepatic toxicity in rats.
TLDR
The histopathological evaluation of the liver revealed that Hibiscus pigments reduced the incidence of liver lesions including inflammatory, leucocyte infiltration, and necrosis induced by t-BHP in rats, leading to speculation that HAs may play a role in the prevention of oxidative damage in living systems. Expand
Anthocyanins in blue berries of Vaccinium padifolium
TLDR
Anthocyanidin disaccharides have not been identified in the genus Vaccinium previously, and the 3-sambubiosides of peonidin, petunidin and malvidin are novel compounds. Expand
Antioxidant power quantification of decoction and cold infusions of Hibiscus sabdariffa flowers
Abstract In this paper the overall antioxidant power, expressed as Briggs–Rauscher antioxidant index, of decoction or cold infusions of dried Hibiscus sabdariffa flowers was determined at 25 and 37Expand
Characterization, quantification, and bioactivities of anthocyanins in Cornus species.
TLDR
In cyclooxygenase (COX)-I and -II enzyme inhibitory assays, anthocyanins 1-3 showed activities of 9.2 and 11.7%; 7.6 and 12.4%; and 5.3 and 7.8%, respectively, compared to Naproxen and ibuprofen, which are high levels of antioxidant activity. Expand
Induction of apoptosis by hibiscus protocatechuic acid in human leukemia cells via reduction of retinoblastoma (RB) phosphorylation and Bcl-2 expression.
TLDR
Data suggest that Hibiscus PCA is an apoptosis inducer in human leukemia cells, and that RB phosphorylation and Bcl-2 protein may play a crucial role in the early stage. Expand
The anthocyanidins cyanidin and delphinidin are potent inhibitors of the epidermal growth-factor receptor.
TLDR
The anthocyanidins cy and del are potent inhibitors of the EGFR, shutting off downstream signaling cascades, which might contribute substantially to the growth-inhibitory properties of these natural food constituents. Expand
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