Preparative Native Continuous Polyacrylamide Gel Electrophoresis (PNC‐PAGE): An Efficient Method for Isolating Cadmium Cofactors in Biological Systems

@article{Kastenholz2004PreparativeNC,
  title={Preparative Native Continuous Polyacrylamide Gel Electrophoresis (PNC‐PAGE): An Efficient Method for Isolating Cadmium Cofactors in Biological Systems},
  author={B. Kastenholz},
  journal={Analytical Letters},
  year={2004},
  volume={37},
  pages={657 - 665}
}
  • B. Kastenholz
  • Published 2004
  • Chemistry
  • Analytical Letters
  • Abstract A new electrophoretic method [preparative native continuous polyacrylamide gel electrophoresis (PNC‐PAGE)] was developed to isolate cadmium cofactor‐containing proteins in biological systems. For this purpose Arabidopsis cytosol was subjected to gel permeation chromatography (GPC) and high molecular mass cadmium proteins (MW ≈ 200 kDa) in a GPC fraction of this plant were isolated by PNC‐PAGE. Furthermore plant cytosol was directly separated by this method. The cadmium concentrations… CONTINUE READING
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