Preparation of3H-phosphatidylmyoinositol withKloeckera brevis

Abstract

The growth ofKloeckera brevis in the presence of uniformly tritiated myoinositol is described. The major radioactive lipid isolated from the cells is3H-phosphatidylmyoinositol, labeled exclusively in the myoinositol moiety. Chromatographic methods for purifying3H-phosphatidylmyoinositol, free from other labeled lipids, are described, and the use of this… (More)
DOI: 10.1007/BF02531309

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