Preparation of zebrafish embryos for transmission electron microscopy.


INTRODUCTIONThis protocol describes a procedure for the fixation and embedding of zebrafish embryos at organogenesis stages (48-72 hours post-fertilization [hpf]) for transmission electron microscopy (TEM). The lengths of individual steps may be adjusted according to the developmental stage of the specimen. 
DOI: 10.1101/pdb.prot4772