Preparation of unilamellar liposomes of intermediate size (0.1-0.2 mumol) by a combination of reverse phase evaporation and extrusion through polycarbonate membranes.

@article{Szoka1980PreparationOU,
  title={Preparation of unilamellar liposomes of intermediate size (0.1-0.2 mumol) by a combination of reverse phase evaporation and extrusion through polycarbonate membranes.},
  author={Francis C. Szoka and F R Olson and Trevor J. Heath and W. J. Vail and Eric Mayhew and Demetrios Papahadjopoulos},
  journal={Biochimica et biophysica acta},
  year={1980},
  volume={601 3},
  pages={559-71}
}
Liposomes can be prepared by a combination of reverse phase evaporation and sequential extrusion through polycarbonate membranes. The vesicles have diameters in the range 0.05-0.5 micron and are mostly unilamellar as indicated by electon microscopy, capture volume, and availability of reactive groups to periodate oxidation. Sequential extrusion leads to a decrease in the encapsulation efficiency by 2-4-fold, depending upon the lipid composition. The inclusion of cholesterol at a 1 : 1 molar… CONTINUE READING

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