Preparation of the epsilon subunit and epsilon subunit-deficient chloroplast coupling factor 1 in reconstitutively active forms.


A simple and rapid method is described for releasing the epsilon subunit from chloroplast coupling factor 1 by treatment with 20% ethanol on an anion exchange column. The resulting epsilon subunit-deficient enzyme is a permanently active Ca2+-ATPase, but an inactive coupling factor. Recombination with the epsilon subunit returns the enzyme to the latent Ca2… (More)


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