Corpus ID: 25931648

Preparation of soluble murine IL-6 receptor and anti-murine IL-6 receptor antibodies.

  title={Preparation of soluble murine IL-6 receptor and anti-murine IL-6 receptor antibodies.},
  author={T. Saito and K. Yasukawa and H. Suzuki and K. Futatsugi and T. Fukunaga and C. Yokomizo and Y. Koishihara and H. Fukui and Y. Ohsugi and H. Yawata},
  journal={Journal of immunology},
  volume={147 1},
Starting with a previously isolated cDNA clone encoding murine IL-6R, a stable transformed Chinese hamster ovary cell line constitutively expressing soluble murine IL-6R (smIL-6R) has been established. The smIL-6R was purified to homogeneity by sequential filtration and chromatography of culture medium. The smIL-6R augmented the sensitivity of M1 cells to IL-6 in their growth inhibition in a dose-response manner. Rat hybridomas producing mAb specific to murine IL-6R were also established. One… Expand
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