Preparation of small RNA libraries for high-throughput sequencing.

@article{Malone2012PreparationOS,
  title={Preparation of small RNA libraries for high-throughput sequencing.},
  author={Colin D. Malone and J. Brennecke and Ben Czech and A. A. Aravin and Gregory J Hannon},
  journal={Cold Spring Harbor protocols},
  year={2012},
  volume={2012 10},
  pages={1067-77}
}
This protocol details the process of small RNA cloning for sequencing on the Illumina/Solexa sequencing platform, but it can be easily modified for use on other next-generation platforms (e.g., SOLiD, 454). This procedure is designed to clone canonical small RNA molecules with 5'-monophosphate and 3'-hydroxyl termini. Modifications, such as the presence of… CONTINUE READING