Preparation of shadowed nuclear envelopes from Xenopus oocyte germinal vesicles for electron microscopy.

Abstract

Methods for examining the structure of the nuclear envelope of oocytes of Xenopus laevis by electron microscopy using metal shadowing have been developed and evaluated. Minor modifications were made to existing methods for preparing specimens by freeze drying, mainly to eliminate unnecessary steps and a rapid method for examining the structure and… (More)

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