Preparation of reduced representation bisulfite sequencing libraries for genome-scale DNA methylation profiling

@article{Gu2011PreparationOR,
  title={Preparation of reduced representation bisulfite sequencing libraries for genome-scale DNA methylation profiling},
  author={Hongcang Gu and Z. Smith and C. Bock and P. Boyle and A. Gnirke and A. Meissner},
  journal={Nature Protocols},
  year={2011},
  volume={6},
  pages={468-481}
}
  • Hongcang Gu, Z. Smith, +3 authors A. Meissner
  • Published 2011
  • Biology, Medicine
  • Nature Protocols
  • Genome-wide mapping of 5-methylcytosine is of broad interest to many fields of biology and medicine. A variety of methods have been developed, and several have recently been advanced to genome-wide scale using arrays and next-generation sequencing approaches. We have previously reported reduced representation bisulfite sequencing (RRBS), a bisulfite-based protocol that enriches CG-rich parts of the genome, thereby reducing the amount of sequencing required while capturing the majority of… CONTINUE READING
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