Preparation of recombinant argininosuccinate synthetase and argininosuccinate lyase: expression of the enzymes in rat tissues.

@article{Yu1995PreparationOR,
  title={Preparation of recombinant argininosuccinate synthetase and argininosuccinate lyase: expression of the enzymes in rat tissues.},
  author={Ying Jie Yu and Kazutoyo Terada and Akitoshi Nagasaki and Masaki Takiguchi and Masataka Mori},
  journal={Journal of biochemistry},
  year={1995},
  volume={117 5},
  pages={952-7}
}
Nitric oxide (NO) is synthesized from arginine by nitric oxide synthase, generating citrulline as another product, which can be recycled to arginine by argininosuccinate synthetase and argininosuccinate lyase. Rat argininosuccinate synthetase was expressed in Escherichia coli as a fusion protein with maltose binding protein, cleaved from binding protein, and purified. The purified synthetase had no enzyme activity. Rat argininosuccinate lyase was expressed in E. coli using pET-3a as a vector… CONTINUE READING
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