Preparation of peptide-MHC and T-cell receptor dextramers by biotinylated dextran doping.

@article{Bethune2017PreparationOP,
  title={Preparation of peptide-MHC and T-cell receptor dextramers by biotinylated dextran doping.},
  author={Michael T. Bethune and Begonya Comin-Anduix and Yu-Hsien Hwang Fu and Antoni Ribas and David Baltimore},
  journal={BioTechniques},
  year={2017},
  volume={62 3},
  pages={123-130}
}
Peptide-major histocompatibility complex (pMHC) multimers enable the detection, characterization, and isolation of antigen-specific T-cell subsets at the single-cell level via flow cytometry and fluorescence microscopy. These labeling reagents exploit a multivalent scaffold to increase the avidity of individually weak T-cell receptor (TCR)-pMHC interactions. Dextramers are an improvement over the original streptavidin-based tetramer technology because they are more multivalent, improving… CONTINUE READING

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