Preparation of nonhistone chromosomal phosphoproteins

Stimulation of confluent monolayers of nondividing WI-38 cells to proliferate is accompanied by a dot~bling in the rate of phosphorylation of nonhistone chromosomal proteins during the first hour. The use of cycloheximide to inhibit protein synthesis does not appear to affect phosphorylation of the nonhistones. Nondividing and serum-stimulated cells exhibit… (More)