Preparation of monoclonal antibodies to C3b by immunization with C3b(i)-sepharose.

  title={Preparation of monoclonal antibodies to C3b by immunization with C3b(i)-sepharose.},
  author={Lj Tosi{\'c} and William M. Sutherland and John B. Kurek and Jeffrey C. Edberg and Ronald P Taylor},
  journal={Journal of immunological methods},
  volume={120 2},
We have prepared and characterized four monoclonal antibodies (MAbs) to human C3b of high specificity and affinity. Our procedure did not require a purified source of C3b for immunization. Instead, C3b and C3bi were deposited on Sepharose 4B via the alternative pathway of complement activation in normal human serum, and this C3b(i)-Sepharose served as the immunogen. C3b(i)-Sepharose was also prepared from a number of primate and non-primate sources, and this allowed us to demonstrate that the… CONTINUE READING

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