Preparation of monoclonal antibodies against acid alpha-D-glucosidase for study of Chinese glycogenosis type II patients.

@article{Lin1992PreparationOM,
  title={Preparation of monoclonal antibodies against acid alpha-D-glucosidase for study of Chinese glycogenosis type II patients.},
  author={C Y Lin and Sang Yeol Lee and Z N Chang and S N Su and Betau Hwang and Sheng Hong Han},
  journal={Hybridoma},
  year={1992},
  volume={11 4},
  pages={493-505}
}
Two monoclonal antibodies (Mabs), 8-23 and 4-6, against human acid alpha-D-glucosidase were generated to analyse the intracellular alpha-D-glucosidase from seven Chinese Pompe's disease families with the following study design: [1] Purified alpha-D-glucosidase from normal human urine was used as antigen for immunization of mice. [2] The splenic cells of immunized mice were isolated and fused with myeloma cells NS-1 for generation of hybridomas and production of anti-human alpha-D-glucosidase… CONTINUE READING