Preparation of monoclonal antibodies against NS1 of avian influenza virus and the antigenic epitope identification

@inproceedings{Xiurong2013PreparationOM,
  title={Preparation of monoclonal antibodies against NS1 of avian influenza virus and the antigenic epitope identification},
  author={Wang Xiu-rong},
  year={2013}
}
To prepare monoclonal antibodies(MAb) against NS1 of avian influenza virus(AIV),BALB/c mice were immunized with purified recombinant NS1 expressed in E.coli.The immunized mice spleen cells were fused with SP2/0,and then 2 MAbs(D7 and D9) against NS1 were screened by indirect ELISA.The 2 MAbs were belonging to IgG1 subclass and κ light chain.Western blot assay showed that the MAbs were reacted with recombinant NS1.Indirect immunofluorescence assay indicated that the MAbs reacted with NS1… CONTINUE READING

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