Preparation of ligation intermediates and related polynucleotide pyrophosphates.

@article{Chu1984PreparationOL,
  title={Preparation of ligation intermediates and related polynucleotide pyrophosphates.},
  author={Byron C. H. Chu and Leslie E. Orgel},
  journal={Biochimica et biophysica acta},
  year={1984},
  volume={782 1},
  pages={103-5}
}
Unprotected oligonucleotides and oligodeoxynucleotides terminated with an unhindered 5'-phosphate group react with nucleoside 5'- phosphorimidazolides in aqueous solution to give 'capped' pyrophosphates in at least 70% yield. If adenosine 5'- phosphorimidazolide is used as a substrate in the reaction, ligase intermediates are obtained as products.