Preparation of leptin antagonists by site-directed mutagenesis of human, ovine, rat, and mouse leptin's site III: implications on blocking undesired leptin action in vivo.

@article{Solomon2006PreparationOL,
  title={Preparation of leptin antagonists by site-directed mutagenesis of human, ovine, rat, and mouse leptin's site III: implications on blocking undesired leptin action in vivo.},
  author={Gili Solomon and Leonora Niv-Spector and Dana Gonen-Berger and Isabelle Callebaut and Jean Djiane and Françoise R{\'e}villion},
  journal={Annals of the New York Academy of Sciences},
  year={2006},
  volume={1091},
  pages={
          531-9
        }
}
Six muteins of human, ovine, rat, and mouse leptins mutated to Ala in amino acids 39-41 or 39-42 were prepared by site-directed mutagenesis of the putative site III, which does not affect binding but is necessary for receptor activation, then expressed, solubilized in 4.5 M urea, at pH 11.3 in presence of cysteine, refolded and purified to homogeneity by anion-exchange chromatography on Q-Sepharose or combination of anion-exchange chromatography followed by gel filtration. The overall yields… CONTINUE READING
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