Preparation of large numbers of plasmid DNA samples in microtiter plates by the alkaline lysis method.

@article{Gibson1987PreparationOL,
  title={Preparation of large numbers of plasmid DNA samples in microtiter plates by the alkaline lysis method.},
  author={Toby J. Gibson and John E. Sulston},
  journal={Gene analysis techniques},
  year={1987},
  volume={4 3},
  pages={41-4}
}
A protocol is described for the growth and preparation of plasmid DNAs from small culture volumes (250 microliters) and utilizing standard 96-well plates. Several hundred plasmids can be prepared simultaneously, yielding sufficient DNA for subsequent analysis by restriction digestion and gel electrophoresis. This protocol may be useful for rapid screening of clones arising in recombinant DNA work such as site-directed mutagenesis, oligonucleotide cassette cloning, deletion analysis, etc. The… CONTINUE READING

From This Paper

Topics from this paper.

Citations

Publications citing this paper.
Showing 1-3 of 3 extracted citations

E. coli Plasmid Vectors

Methods in Molecular Biology™ • 2003
View 10 Excerpts
Highly Influenced

Detection of putatively thermophilic anaerobic methanotrophs ( ANMEs ) in 1 diffuse hydrothermal vent fluids 2 3

Alexander Y. Merkela, Julie A Huber, Nikolay A. Chernyha, Elizaveta A. Bonch-Osmolovskayaa, Alexander V. Lebedinskya
2012
View 1 Excerpt

Similar Papers

Loading similar papers…