Preparation of large RNA oligonucleotides with complementary isotope-labeled segments for NMR structural studies

@article{Tzakos2007PreparationOL,
  title={Preparation of large RNA oligonucleotides with complementary isotope-labeled segments for NMR structural studies},
  author={Andreas G Tzakos and Laura E. Easton and Peter J. Lukavsky},
  journal={Nature Protocols},
  year={2007},
  volume={2},
  pages={2139-2147}
}
RNA structure determination by solution NMR spectroscopy is often restricted to small RNAs (<15 kDa) owing to the problem of chemical shift degeneracy. A fruitful coupling of novel NMR techniques with segmental RNA labeling methodologies could be a powerful tool to overcome the molecular mass limitation of RNA NMR spectroscopy. Herein, we describe a time… CONTINUE READING