Preparation of isotopically labeled ribonucleotides for multidimensional NMR spectroscopy of RNA.

Abstract

A general method for large scale preparation of uniformly isotopically labeled ribonucleotides and RNAs is described. Bacteria are grown on isotopic growth medium, and their nucleic acids are harvested and degraded to mononucleotides. These are enzymatically converted into ribonucleoside triphosphates, which are used in transcription reactions in vitro to… (More)

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@article{Batey1992PreparationOI, title={Preparation of isotopically labeled ribonucleotides for multidimensional NMR spectroscopy of RNA.}, author={Robert T Batey and Mitsuo Inada and Elizabeth B Kujawinski and Joseph D. Puglisi and Jeffrey Williamson}, journal={Nucleic acids research}, year={1992}, volume={20 17}, pages={4515-23} }