Preparation of intracellular proteins from a white-rot fungus surrounded by polysaccharide sheath and optimization of their two-dimensional electrophoresis for proteomic studies.

Abstract

The functions and properties of fungal sheath, an extracellular polysaccharide produced by many white-rot fungi, have been studied. However, the strong adherence of the sheath to fungal hyphae had been a major impediment in preparing intracellular proteins from the fungi and analyzing their cellular responses. To overcome this issue, we developed a rapid… (More)
DOI: 10.1016/j.mimet.2017.09.009

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Cite this paper

@article{Watanabe2017PreparationOI, title={Preparation of intracellular proteins from a white-rot fungus surrounded by polysaccharide sheath and optimization of their two-dimensional electrophoresis for proteomic studies.}, author={Takahito Watanabe and Koichi Yoshioka and Ayako Kido and Junseok Lee and Hikari Akiyoshi and Takashi Watanabe}, journal={Journal of microbiological methods}, year={2017}, volume={142}, pages={63-70} }