Preparation of interstitial lung cells by enzymatic digestion of tissue slices: preliminary characterization by morphology and performance in functional assays.

@article{Holt1985PreparationOI,
  title={Preparation of interstitial lung cells by enzymatic digestion of tissue slices: preliminary characterization by morphology and performance in functional assays.},
  author={Patrick G. Holt and A M Degebrodt and Thierry J. Venaille and C T O'Leary and Karen D. Krska and James Patrick Flexman and Helen B Farrell and Geoffrey R. Shellam and Peter Young and J Penhale},
  journal={Immunology},
  year={1985},
  volume={54 1},
  pages={
          139-47
        }
}
A technique is reported here for the quantitative extraction of live cells from the lung interstitium; it involves the incubation of slices of perfused lung in a mixture containing optimal concentrations of collagenase, DNAse, and fetal calf serum, followed by the simultaneous recovery and fractionation of cells released from the tissue matrix on a six-step discontinuous percoll gradient. Yields in the order of 10(8) viable cells per gram of lung were routinely achieved with tissues from rat… CONTINUE READING
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