[Preparation of inside-out vesicles from red blood cell membranes and measurement for its Ca2+ uptake].

  • Hui-yuan Luo
  • Published 1990 in
    Zhongguo yi xue ke xue yuan xue bao. Acta…

Abstract

Sealed, inside-out red cell membrane vesicles (IOV) from rats were prepared by a modified method of Steck (1974). The preparation contained 70% of IOV. In the presence of ATP and Mg2+, these vesicles actively took up Ca2+ with a high efficiency and reproducibility. The intraassay coefficient of variance (C.V.) was 3.6% and the interassay C.V. was 13.58%. The active Ca2+ transport reached the maximal level in 10 minutes of incubation, and linearly correlated with the IOV concentration. The Ca2+ uptake rate in Wistar rats was 9.39 +/- 1.28 nmol/mg IOV protein/min. IOV rapidly lost Ca2+ when A23187 was added. The active transport was stimulated by calmodulin, and inhibited by trifluoperazine.

Cite this paper

@article{Luo1990PreparationOI, title={[Preparation of inside-out vesicles from red blood cell membranes and measurement for its Ca2+ uptake].}, author={Hui-yuan Luo}, journal={Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae}, year={1990}, volume={12 1}, pages={70-3} }