Preparation of homogeneous samples of double-labelled protein suitable for single-molecule FRET measurements.

Abstract

Preparation of pure and homogenous site specifically single- and double-labelled biopolymers suitable for spectroscopic determination of structural characteristics is a major current challenge in biopolymers chemistry. In particular, proper analysis of single-molecule Förster resonance energy transfer measurements is based on the spectral characteristics of… (More)
DOI: 10.1007/s00216-013-7002-2

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