Preparation of hepatic gap (communicating) junctions. Identification of the constituent polypeptide subunits.

@article{Culvenor1977PreparationOH,
  title={Preparation of hepatic gap (communicating) junctions. Identification of the constituent polypeptide subunits.},
  author={Janetta Gladys Culvenor and W. Howard Evans},
  journal={The Biochemical journal},
  year={1977},
  volume={168 3},
  pages={475-81}
}
1. Gap (communicating) junctions are plasma-membrane specializations of characteristic morphology that form transmembrane channels allowing direct communication between cells. Their preparation is described starting from mouse liver plasma membranes and the constituent polypeptides are deduced. 2. Gap junctions co-purify with collagen fibres when the plasma-membrane residues insoluble in N-dodecyl sarcosinate are fractionated on sucrose gradients. Sucrose-density perturbation by relipidation of… CONTINUE READING