Preparation of genomic DNA from Dictyostelium discoideum for PCR analysis.

@article{Charette2004PreparationOG,
  title={Preparation of genomic DNA from Dictyostelium discoideum for PCR analysis.},
  author={S J Charette and Pierre Cosson},
  journal={BioTechniques},
  year={2004},
  volume={36 4},
  pages={
          574-5
        }
}
The soil amoeba Dictyostelium discoideum is a practical genetic system in which targeted gene disruption by homologous recombination allows the study of specific gene functions (1–3). The efficiency of homologous recombination, however, can be less than 1%, and the identification of the desired clones requires the analysis of the genotype of many individual clones. However, the standard protocols to prepare genomic DNA for genotyping require a large number of cells (>107) and also necessitate… CONTINUE READING
19 Citations
0 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 19 extracted citations

Similar Papers

Loading similar papers…