Preparation of freeze-dried cryosections for quantitative X-ray microanalysis of electrolytes in biological soft tissues

@article{Drge1978PreparationOF,
  title={Preparation of freeze-dried cryosections for quantitative X-ray microanalysis of electrolytes in biological soft tissues},
  author={Adolph D{\"o}rge and Roger Rick and Kalle Gehring and Klaus Thurau},
  journal={Pfl{\"u}gers Archiv},
  year={1978},
  volume={373},
  pages={85-97}
}
A procedure is described which allows the evaluation of wet weight concentrations of diffusible substances in biological soft tissue on a cellular level by the use of energy dispersive X-ray microanalysis. Epithelia of frog skin and toad urinary bladder were used to prepare freeze-dried cryosections without the use of chemical fixatives, cryoprotectants, floating solutions or coating materials. The specimens were shock-frozen inl-propane (−180°C), cryosectioned (−80°C), sandwiched between… CONTINUE READING