Preparation of extended metaphase chromosomes from human cultured cells using a topoisomerase II inhibitor, ICRF-193.

@article{Kohda2001PreparationOE,
  title={Preparation of extended metaphase chromosomes from human cultured cells using a topoisomerase II inhibitor, ICRF-193.},
  author={Atsushi Kohda and Hiroshi Taguchi and Katsuzumi Okumura},
  journal={Bioscience, biotechnology, and biochemistry},
  year={2001},
  volume={65 5},
  pages={1248-51}
}
Effects of ICRF-193, a topoisomerase II inhibitor, on metaphase chromosome preparations were examined. A short-time exposure of this drug to human HL60 cells in a suspension culture before harvest resulted in obtaining more extended metaphase chromosomes. The length of chromosome 6 identified by fluorescence in situ hybridization was twice as long with this drug treatment. Together with effectiveness for adherent HepG2 cells, these results suggest that treatments with ICRF-193 provide a simple… CONTINUE READING