Preparation of extended in vitro cultures of bovine hepatocytes that are hormonally responsive.

@article{Donkin1993PreparationOE,
  title={Preparation of extended in vitro cultures of bovine hepatocytes that are hormonally responsive.},
  author={Shawn S. Donkin and Louis E. Armentano},
  journal={Journal of animal science},
  year={1993},
  volume={71 8},
  pages={2218-27}
}
Hepatocytes isolated from male dairy calves were used in monolayer culture or in suspension culture to determine their suitability for the study of hormonal regulation of hepatic gluconeogenesis. The rate of gluconeogenesis (nanomoles of 2.5 mM [2-14C]propionate incorporated into glucose.microgram of DNA-1.hour-1) was higher for monolayers than for suspension cultures. Gluconeogenesis and ureagenesis (nanomoles of urea N formed.microgram of DNA-1.3 hours-1) were similar in monolayers cultured… CONTINUE READING