Preparation of enzymatically active recombinant class III protein deacetylases.

@article{North2005PreparationOE,
  title={Preparation of enzymatically active recombinant class III protein deacetylases.},
  author={Brian J North and Bj{\"o}rn Schwer and Nidhi Ahuja and B. Marshall and Eric Verdin},
  journal={Methods},
  year={2005},
  volume={36 4},
  pages={338-45}
}
Class III histone deacetylases, or sirtuins, are homologous to the Saccharomyces cerevisiae transcriptional regulator SIR2. The class III enzymes are characterized by their dependence on nicotinamide adenine dinucleotide (NAD+). This cofactor serves as an acetyl-group acceptor in the deacetylation reaction generating O-acetyl-ADP-ribose. Enzymatic activity of sirtuin can be measured in vitro using recombinant proteins purified from mammalian cells after overexpression or after purification from… CONTINUE READING

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