Preparation of dopamine 3-O-sulphate and dopamine 4-O-sulphate as reference substances and high-performance liquid chromatographic trace determination.

Abstract

Simple syntheses of the biologically important but hitherto difficult to obtain dopamine sulpho conjugates dopamine 3-O-sulphate (I) and dopamine 4-O-sulphate (II), as analytical reference substances, starting from dopamine hydrochloride are described. A method for the determination of I and II with reversed-phase high-performance liquid chromatographic separation and coulometric detection in human urine together with calibration and current-voltage curves are presented. Detection limits of approximately 100 pg of I or II and unequivocal substance identifications even in very complex substrates such as human urine are reported.

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@article{Kienzl1988PreparationOD, title={Preparation of dopamine 3-O-sulphate and dopamine 4-O-sulphate as reference substances and high-performance liquid chromatographic trace determination.}, author={Elisabeth Kienzl and Konrad Eichinger}, journal={Journal of chromatography}, year={1988}, volume={430 2}, pages={263-9} }