Preparation of cytoplasmic RNA from tissue culture cells.

  title={Preparation of cytoplasmic RNA from tissue culture cells.},
  author={Michael Gilman},
  journal={Current protocols in molecular biology},
  volume={Chapter 4},
  pages={Unit 4.1}
The protocol described in this unit is a fast and streamlined procedure for preparing total cytoplasmic RNA from many cultures simultaneously for nuclease protection analysis. It is scaled for small cultures--1 to 2 dishes of adherent cells or 10 to 20 ml of a suspension culture. The procedure works well for many cell types. If full-length RNA is required, ribonuclease inhibitors should be added to the lysis buffer (as described in this unit) or the guanidinium isothiocyanate method should be… CONTINUE READING

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