Preparation of chitin nanofibers with a uniform width as alpha-chitin from crab shells.


Chitin nanofibers were prepared from dried crab shells by a simple grinding treatment in a never-dried state under an acidic condition after the removal of proteins and minerals. The obtained nanofibers were observed by FE-SEM and found to have a uniform width of approximately 10-20 nm and high aspect ratio; both these findings were similar to those for nanofibers from prawns. Furthermore, it was confirmed that the nanofibers were extracted from the natural chitin/protein/mineral composites of crab shell in their original state. That is, the N-acetyl group was not removed and the alpha-chitin crystal structure was maintained, as confirmed by elemental analysis data, FT-IR spectra, and X-ray diffraction profiles.

DOI: 10.1021/bm900163d
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@article{Ifuku2009PreparationOC, title={Preparation of chitin nanofibers with a uniform width as alpha-chitin from crab shells.}, author={Shinsuke Ifuku and Masaya Nogi and Kentaro Abe and Masafumi Yoshioka and Minoru Morimoto and Hiroyuki Saimoto and Hiroyuki Yano}, journal={Biomacromolecules}, year={2009}, volume={10 6}, pages={1584-8} }