Preparation of bacteriophage λ DNA using the TL-100 ultracentrifuge

  title={Preparation of bacteriophage λ DNA using the TL-100 ultracentrifuge},
  author={Michael F. Buckley and James W. Goding},
Abstract A procedure for the preparation of DNA from bacteriophage λ is described, using the Beckman TL-100 bench-top ultracentrifuge. The procedure involves growth of phage in agar plates, precipitation with polyethylene glycol, and a single centrifugation in cesium chloride under conditions that disrupt the phage coat. The method avoids the use of enzymes, ion exchange resins, and phenol. It can be completed in less than a day. The resulting DNA is good purity and is easily cuttable by… CONTINUE READING