Preparation of artificial bifunctional enzymes by gene fusion.

  title={Preparation of artificial bifunctional enzymes by gene fusion.},
  author={Leif B{\"u}low},
  journal={Biochemical Society symposium},
  • Leif Bülow
  • Published 1990 in Biochemical Society symposium
Within the cellular framework operate a number of sequentially acting enzyme systems of varying complexity and spatial organization. In some cases they are found as bi- or even multi-functional enzymes, where each single polypeptide chain carries two or more catalytic functions. To mimic these enzymes, a number of different bifunctional enzymes have been prepared by in-frame gene fusion. Thus, beta-galactosidase and galactokinase as well as beta-galactosidase and galactose dehydrogenase were… CONTINUE READING

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