Preparation of an inner membrane-matrix fraction from yeast mitochondria

@article{Velours1977PreparationOA,
  title={Preparation of an inner membrane-matrix fraction from yeast mitochondria},
  author={J. Velours and B. Gu{\'e}rin and M. Duvert},
  journal={Archives of Biochemistry and Biophysics},
  year={1977},
  volume={182},
  pages={295-304}
}
Abstract Treatment of yeast mitochondria with digitonin was used in order to prepare an inner membrane-matrix fraction preserving its permeability properties. The incubation time of mitochondria with digitonin was an essential parameter for the selective solubilization of the outer membrane. The incubation of mitochondria for l min at different concentrations of digitonin led to a three-step release of mitochondrial enzymes: (a) at low concentrations of digitonin, adenylate kinase was released… Expand
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