Preparation of adsorbents for affinity chromatography using TSKgel Tresyl-Toyopearl 650M.

Abstract

The optimum conditions for the coupling of proteins were investigated using TSKgel Tresyl-Toyopearl 650M. They were dependent on the proteins coupled. For example, when soybean trypsin inhibitor was coupled at pH 8 the coupling was completed within 1 h and the subsequent adsorption capacity for trypsin was maximal. Longer coupling times decreased the… (More)

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@article{Nakamura1989PreparationOA, title={Preparation of adsorbents for affinity chromatography using TSKgel Tresyl-Toyopearl 650M.}, author={Kenji Nakamura and Kazunori Toyoda and Yoshihisa Kato and Kiyohito Shimura and Ken Ichi Kasai}, journal={Journal of chromatography}, year={1989}, volume={478 1}, pages={159-67} }