Preparation of a whole-cell biocatalyst of Aspergillus niger lipase and its practical properties.

Abstract

Aspergillus niger lipase (ANL), a widely used hydrolase, was displayed for the first time on the surface of Saccharomyces cerevisiae using a-agglutinin as an anchor protein. Localization of ANL on the cell surface was confirmed by immunofluorescence microscopy. The displayed ANL was confirmed to be active toward tributyrin and p-nitrophenyl caprylate (pNPC… (More)
DOI: 10.1021/jf1008555

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