Preparation of Refractile Spores of Clostridium thermosaccharolyticum Involves a Solventogenic Phase.

@article{Landuyt1992PreparationOR,
  title={Preparation of Refractile Spores of Clostridium thermosaccharolyticum Involves a Solventogenic Phase.},
  author={S L Landuyt and En-Ching Hsu},
  journal={Applied and environmental microbiology},
  year={1992},
  volume={58 6},
  pages={1797-800}
}
Conversion of vegetative cells of Clostridium thermosaccharolyticum to refractile endospores was achieved by sequential transfer and dilution at each generation, with a final dilution into a sporulation medium that contained xylan supplemented with excess calcium. The subsequent growth was synchronous and resulted in elongated, solventogenic cells that were then shifted to 35 degrees C to permit further differentiation without cell division. The synchronized cells grown in xylan medium… CONTINUE READING

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